27 UPPER JAMES
| ROUTE TYPE | LOCAL |
| DIRECTION | NORTH – SOUTH |
| SERVICE TYPE | YEAR-ROUND, DAILY |
| OPERATING HOURS | Weekdays: 5:30am – 1:20am Saturday: 5:30am – 1am Sunday: 6am – 12am |
| FREQUENCY | Weekdays – Peak: 15 mins / off-peak 20 mins / evening: 30 mins Saturday – morning: 30 mins / day: 20 mins / evening: 30 mins Sunday – morning: 60 mins / day: 30 mins / evening: 60 mins |
This route travels from downtown Hamilton to the south, looping at the Mountain Transit Centre in Glanbrook.
| North | MacNab Transit Terminal | |
| South | Mountain Regional Transit Centre | |
| PHOTOS: | ||
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This photo was taken of HSR 7718 running as a Route 27 Upper James at Gore Park. | |
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This photo was taken April 18, 2007 of HSR 9710 running as a Route 27 Upper James at the corner of Main St W and James St S. | |
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This photo was taken May 16, 2007 of HSR 9920 running as a Route 27 Upper James at the corner of Jackson St W and James St S. | |
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This photo was taken April 13, 2007 of HSR 510502 running as a Route 27 Upper James at the corner of Jackson St W and James St S. | |
Westbound 7 LOCKE will be detoured due to Charlton closure due to hydro pole installation on June 27th from 9:00 AM to 4:00 PM. Details: 
Lift Bridge Closure Detours 11 PARKDALE nights of June 12th - 17th and June 19th - 24th from 8:00 PM - 5:00 AM. Details: 
22 UPPER OTTAWA and Route 43 STONE CHURCH will be detoured for Dartnall/Stone Church intersection closure from June 21st at 9:00 AM to June 28th. Details: 
35 COLLEGE is detoured for Garth and Rymal intersection closure Wednesday, June 21st, 2017 from about 7:00 AM to 6:00 PM. Details: 
